Alternative Names:

aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685

Application:

WB

Reactivity:

H M R Mk Ch Ze Fish

Dilution:

WB 1:5000~1:20000

Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat

Purification:

Affinity-chromatography

Specificity:

HRP Anti-GAPDH antibody detects endogenous levels of total GAPDH

Immunogen:

A synthesized peptide derived from human HRP Anti-GAPDH antibody

Description:

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.