Mol Weight : 42kDa

UniProt : P68133

Isotype : Rabbit IgG

Clonality   : Monoclonal

Alternative Names:

a actin; ACTA; ACTA1; Actin alpha skeletal muscle; Actin; actina; actine; aktin; Alpha Actin 1; Alpha skeletal muscle Actin; ASMA;   CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat Chicken

Dilution:

WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

Western blot analysis of Actin expression in (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate.

Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody.

Immunofluorescent analysis of Hela cells, using Actin Antibody.

Purification:

Affinity-chromatography

Specificity:

Actin Antibody detects endogenous levels of total actin protein.

Immunogen:

A synthesized peptide derived from human Actin.

Description:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.